Proteolytic selection for protein folding using filamentous bacteriophages.


BACKGROUND Filamentous bacteriophages have been used for the selection of folded peptide and protein 'ligands' by binding the phage to 'receptor'-coated solid phase. Here, using proteolysis, we have developed a technique for the selection of folded and stable proteins that is independent of their binding activities. RESULTS When a 21-residue peptide… (More)


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