Proteolytic processing of the astrovirus capsid.

  title={Proteolytic processing of the astrovirus capsid.},
  author={Dorsey M. Bass and Shihong Qiu},
  journal={Journal of virology},
  volume={74 4},
To further characterize the nature of proteolytic processing of the astrovirus capsid, we infected Caco-2 cells with a high multiplicity of astrovirus without trypsin in the presence of 5 to 10% fetal calf serum. These infections were characterized by pulse-chase labeling with [35S]Smethionine, electron microscopy, gel electrophoresis of purified viral particles, and analysis of infectivity of such particles with and without added trypsin. Pulse-chase experiments showed that the astrovirus… CONTINUE READING