Proteolytic processing of the alpha-chain of the lysosomal enzyme, beta-hexosaminidase, in normal human fibroblasts.

@article{Little1988ProteolyticPO,
  title={Proteolytic processing of the alpha-chain of the lysosomal enzyme, beta-hexosaminidase, in normal human fibroblasts.},
  author={Lewis E. Little and M. M. Lau and Doris V. K. Quon and Audree V. Fowler and Elizabeth F. Neufeld},
  journal={The Journal of biological chemistry},
  year={1988},
  volume={263 9},
  pages={4288-92}
}
The two subunits of beta-hexosaminidase undergo many post-translational modifications characteristic of lysosomal proteins, including limited proteolysis. To identify proteolytic cleavage sites in the alpha-chain, we have biosynthetically radiolabeled the transient forms, isolated these by immunoprecipitation, gel electrophoresis, and electroelution, and… CONTINUE READING