Proteolytic fragments of anti-HIV and anti-tumor proteins MAP30 and GAP31 are biologically active.

@article{Huang1999ProteolyticFO,
  title={Proteolytic fragments of anti-HIV and anti-tumor proteins MAP30 and GAP31 are biologically active.},
  author={Philip Lin Huang and Ye Sun and Hao Chen and Hsiang Fu Kung and Sylvia Lee-Huang},
  journal={Biochemical and biophysical research communications},
  year={1999},
  volume={262 3},
  pages={615-23}
}
We analyzed the structural and functional organization of anti-HIV and anti-tumor proteins MAP30 and GAP31 by limited proteolysis with endopeptidases Lys-C and Glu-C (V8). MAP30 and GAP31 are resistant to proteolytic digestion under conditions of as much as 5% (w/w) proteases. In the presence of 10% (w/w) protease, the central regions of the proteins are… CONTINUE READING