Proteolytic degradation of sardine (Sardinella gibbosa) proteins by trypsin from skipjack tuna (Katsuwonus pelamis) spleen

@inproceedings{Klomklao2006ProteolyticDO,
  title={Proteolytic degradation of sardine (Sardinella gibbosa) proteins by trypsin from skipjack tuna (Katsuwonus pelamis) spleen},
  author={Sappasith Klomklao and Soottawat Benjakul and Wonnop Visessanguan and Hideki Kishimura and Benjamin K. Simpson},
  year={2006}
}
  • Sappasith Klomklao, Soottawat Benjakul, +2 authors Benjamin K. Simpson
  • Published 2006
  • Chemistry
  • Trypsin from the spleen of skipjack tuna (Katsuwonus pelamis) was purified by ammonium sulfate precipitation and a series of chromatographies, including Sephacryl S-100 and benzamidine-Sepharose 4 fast flow (high sub). The enzyme was purified 22.3-fold with a yield of 51.6%. The molecular weight of trypsin was estimated to be 42 kDa by sodium dodecyl sulfate-polyacrylamide gel electrophoresis. Purified trypsin was able to hydrolyse natural actomyosin (NAM) and myosin, but scarcely hydrolysed… CONTINUE READING

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