Proteolytic cleavage of recombinant type 2A von Willebrand factor mutants R834W and R834Q: inhibition by doxycycline and by monoclonal antibody VP-1.

@article{Tsai1997ProteolyticCO,
  title={Proteolytic cleavage of recombinant type 2A von Willebrand factor mutants R834W and R834Q: inhibition by doxycycline and by monoclonal antibody VP-1.},
  author={Han-Mou Tsai and Ira I. Sussman and David Ginsburg and H Lankhof and Jan J. Sixma and Ronald L. Nagel},
  journal={Blood},
  year={1997},
  volume={89 6},
  pages={1954-62}
}
The susceptibility of recombinant type 2A von Willebrand factor (vWF) to a recently identified plasma metalloproteinase and the potential application of proteolysis inhibition in the treatment of the disease were investigated. Two recombinant type 2A vWF mutants, R834W and R834Q, were spontaneously cleaved by the partially purified plasma proteinase to smaller forms. When treated with guanidine HCI, both the wild-type and the R834W mutant vWF exhibited a biphasic change in proteolytic… CONTINUE READING
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Shear stress enhances the brand disease mutations

  • HM Tsai, II Sussman, RL Nagel
  • J Biol Chem 267:4424,
  • 1992
Highly Influential
4 Excerpts

Subunitplasma protease is dependent on its conformation and requires cal - composition of plasma von Willebrand factor . Cleavage is presentcium

  • HM Tsai, TS Zimmerman, JA Dent, ZM Ruggeri, LH Nannini
  • Blood
  • 1996

RM: tional and Western-blot assessment with special reference to their Matrix metalloproteinase inhibitor BB-94 (batimastat) inhibits hucellular sources in periodontal diseases

  • X Wang, X Fu, PD Brown, MJ Crimmin, Hoffman
  • Ann NY Acad Sci 732:112,
  • 1994
2 Excerpts

Structure and function of human von Willebrandbrand factor and implications for vasoocclusion

  • H Lankhof
  • Blood
  • 1993

Molecular genetics of von Wille - prevent loss of the large multimers is to protect the vWF brand disease

  • D Ginsburg, Bowie EJW
  • Blood
  • 1992

Effects of tetracy in connective tissue remodeling

  • H their inhibitors Ohtani, N Andoh, S Takeha, YT Konttinen
  • FASEB J 5:2145,
  • 1991

Matrix metalloproteinases and their inhibitors Ohtani H , Andoh N , Takeha S , Konttinen YT : Effects of tetracy - in connective tissue remodeling

  • X Wang, X Fu, PD Brown, MJ Crimmin, RM Hoffman
  • FASEB J
  • 1991

constituent subunit

  • S Johansson, B Smedsrod, HM Tsai, RL Nagel, Sussman
  • J Clin Invest
  • 1991

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