Proteolytic activation of a single-chain precursor of hepatocyte growth factor by extracellular serine-protease.

@article{Mizuno1992ProteolyticAO,
  title={Proteolytic activation of a single-chain precursor of hepatocyte growth factor by extracellular serine-protease.},
  author={Keiko Mizuno and Tadamichi Takehara and Tsutomu Nakamura},
  journal={Biochemical and biophysical research communications},
  year={1992},
  volume={189 3},
  pages={1631-8}
}
Hepatocyte growth factor (HGF) is biosynthesized as a single-chain precursor (pro-HGF) and is proteolytically processed to a two-chain mature form. When MRC-5 fibroblasts were pulse-radiolabeled under serum-free conditions, pro-HGF was the predominant molecular form of HGF in the culture medium. CHO cells transfected with an expression plasmid containing a full-size human HGF cDNA produced pro-HGF when these cells were cultured in serum-free medium. These findings suggest that HGF is secreted… CONTINUE READING

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