Proteogenomics of Pristionchus pacificus reveals distinct proteome structure of nematode models.

@article{Borchert2010ProteogenomicsOP,
  title={Proteogenomics of Pristionchus pacificus reveals distinct proteome structure of nematode models.},
  author={Nadine Borchert and Christoph Dieterich and Karsten Krug and W. Sch{\"u}tz and Stephan Jung and A. Nordheim and Ralf J. Sommer and Boris Ma{\vc}ek},
  journal={Genome research},
  year={2010},
  volume={20 6},
  pages={837-46}
}
Pristionchus pacificus is a nematode model organism whose genome has recently been sequenced. To refine the genome annotation we performed transcriptome and proteome analysis and gathered comprehensive experimental information on gene expression. Transcriptome analysis on a 454 Life Sciences (Roche) FLX platform generated >700,000 expressed sequence tags (ESTs) from two normalized EST libraries, whereas proteome analysis on an LTQ-Orbitrap mass spectrometer detected >27,000 nonredundant peptide… CONTINUE READING
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