Proteogenomic analysis of Bradyrhizobium japonicum USDA110 using GenoSuite, an automated multi-algorithmic pipeline.

@article{Kumar2013ProteogenomicAO,
  title={Proteogenomic analysis of Bradyrhizobium japonicum USDA110 using GenoSuite, an automated multi-algorithmic pipeline.},
  author={Dhirendra Kumar and Amit Kumar Yadav and Puneet Kumar Kadimi and Shivashankar H. Nagaraj and Sean M. Grimmond and Debasis Dash},
  journal={Molecular & cellular proteomics : MCP},
  year={2013},
  volume={12 11},
  pages={3388-97}
}
We present GenoSuite, an integrated proteogenomic pipeline to validate, refine and discover protein coding genes using high-throughput mass spectrometry (MS) data from prokaryotes. To demonstrate the effectiveness of GenoSuite, we analyzed proteomics data of Bradyrhizobium japonicum (USDA110), a model organism to study agriculturally important rhizobium-legume symbiosis. Our analysis confirmed 31% of known genes, refined 49 gene models for their translation initiation site (TIS) and discovered… CONTINUE READING
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