Proteins that bind to RNA polymerase II are required for accurate initiation of transcription at the adenovirus 2 major late promoter.

@article{Burton1986ProteinsTB,
  title={Proteins that bind to RNA polymerase II are required for accurate initiation of transcription at the adenovirus 2 major late promoter.},
  author={Zachary F Burton and L G Ortolan and Jack Greenblatt},
  journal={The EMBO journal},
  year={1986},
  volume={5 11},
  pages={2923-30}
}
Extracts prepared from HeLa cell nuclei are inactivated for accurate transcription from the adenovirus 2 major late promoter (Ad2 MLP) by repeated passage over a column containing immobilized calf thymus RNA polymerase II. The capacity for accurate transcription is restored to these extracts by a fraction that is eluted from the RNA polymerase II column with 0.5 M KC1. This fraction contains RNA polymerase II-associating proteins (RAPs). RAPs are not required for the formation of a stable… CONTINUE READING