Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases

  title={Proteins of the Rpf (resuscitation promoting factor) family are peptidoglycan hydrolases},
  author={Miroslav V Telkov and Galina R. Demina and Sergei A. Voloshin and Elena G. Salina and T. V. Dudik and Tatiana N. Stekhanova and Galina V. Mukamolova and K. A. Kazaryan and Anna V. Goncharenko and Michael L Young and Arseny S. Kaprelyants},
  journal={Biochemistry (Moscow)},
The secreted Micrococcus luteus protein, Rpf, is required for successful resuscitation of dormant “non-culturable” M. luteus cells and for growth stimulation in poor media. The biochemical mechanism of Rpf action remained unknown. Theoretical predictions of Rpf domain architecture and organization, together with a recent NMR analysis of the protein structure, indicate that the conserved Rpf domain has a lysozyme-like fold. In the present study, we found that both the secreted native protein and… CONTINUE READING


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