Proteins in the luminal fluid from the bovine oviduct.

  title={Proteins in the luminal fluid from the bovine oviduct.},
  author={Gwilym M. P. Roberts and June M. Parker and H W Symonds},
  journal={Journal of reproduction and fertility},
  volume={45 2},
Oviducal fluid was collected by cannulation from four cows and by irrigation from fifteen slaughtered cows. The proteins in the fluid were examined by polyacrylamide gel electrophoresis at pH 4-5 and pH 8-9, isoelectric focusing on polyacrylamide, immunodiffusion, immunoelectrophoresis, affinity chromatography and gel filtration. The macromolecular components found were mainly serum proteins but small amounts of other proteins were detected in oestrous and dioestrous samples by electrophoresis… CONTINUE READING