Proteinase activity in rumen ciliate protozoa.

  title={Proteinase activity in rumen ciliate protozoa.},
  author={Brett Lockwood and Graham H. Coombs and Alan G. Williams},
  journal={Journal of general microbiology},
  volume={134 9},
Azocasein-degrading proteinase activity was detected in all rumen ciliate protozoa that were examined from four entodiniomorphid and two holotrich genera. All of the activities were optimal in the range pH 4.0-5.0 and were inhibited by cysteine proteinase inhibitors, notably leupeptin. The inhibition profiles and extent of inhibition observed with the different groups of inhibitors were organism-specific. Gelatin-SDS-polyacrylamide gel electrophoresis of protozoal lysates revealed multiple… CONTINUE READING


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