Protein tyrosine phosphatase: enzymatic assays.

@article{Montalibet2005ProteinTP,
  title={Protein tyrosine phosphatase: enzymatic assays.},
  author={Jacqueline Montalibet and Kathryn I Skorey and Brian P. Kennedy},
  journal={Methods},
  year={2005},
  volume={35 1},
  pages={
          2-8
        }
}
Activity assays for tyrosine phosphatases are based on the hydrolysis of a arylphosphate moiety from a synthetic substrate yielding a spectroscopically active product. Many different substrates can be used for these assays with p-nitrophenyl phosphate (pNPP), fluorescein diphosphate (FDP), and 6,8-difluoro-4-methylumbellyferyl phosphate (DiFMUP) being the most efficient and versatile. Equally, larger molecules such as phosphotyrosyl peptides can also be used to mimic more natural substrates… CONTINUE READING
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