Protein turnover analysis in Salmonella Typhimurium during infection by dynamic SILAC, Topograph, and quantitative proteomics.

@article{Wang2016ProteinTA,
  title={Protein turnover analysis in Salmonella Typhimurium during infection by dynamic SILAC, Topograph, and quantitative proteomics.},
  author={Zhe Wang and Qiang-Qiang Han and M. Zhou and Xi Chen and Lin Guo},
  journal={Journal of basic microbiology},
  year={2016},
  volume={56 7},
  pages={
          801-11
        }
}
Protein turnover affects protein abundance and phenotypes. Comprehensive investigation of protein turnover dynamics has the potential to provide substantial information about gene expression. Here we report a large-scale protein turnover study in Salmonella Typhimurium during infection by quantitative proteomics. Murine macrophage-like RAW 264.7 cells were infected with SILAC labeled Salmonella. Bacterial cells were extracted after 0, 30, 60, 120, and 240 min. Mass spectrometry analyses yielded… CONTINUE READING