Protein tryptophan accessibility studied by fluorescence quenching

  title={Protein tryptophan accessibility studied by fluorescence quenching},
  author={Matias N. M{\"o}ller and Ana Denicola},
  journal={Biochemistry and Molecular Biology Education},
  • M. Möller, A. Denicola
  • Published 1 May 2002
  • Physics, Chemistry
  • Biochemistry and Molecular Biology Education
A laboratory class is described to introduce the biochemistry major students to the basic concepts and various applications of fluorescence spectroscopy. Through simple and inexpensive experiments the students learn how to record excitation and emission spectra and measure intrinsic protein fluorescence and its quenching to elucidate the local tryptophan environment. Free tryptophan, ovalbumin, and bovine serum albumin are used for the experiments. The structural information that can be… 
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Topics in fluorescence spectroscopy , Topics in fluorescence spectroscopy , مرکز فناوری اطلاعات و اطلاع رسانی کشاورزی
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