Protein transport across the lung epithelial barrier.

@article{Kim2003ProteinTA,
  title={Protein transport across the lung epithelial barrier.},
  author={Kwang-Jin Kim and Asrar B. Malik},
  journal={American journal of physiology. Lung cellular and molecular physiology},
  year={2003},
  volume={284 2},
  pages={
          L247-59
        }
}
  • Kwang-Jin KimA. Malik
  • Published 1 February 2003
  • Biology
  • American journal of physiology. Lung cellular and molecular physiology
Alveolar lining fluid normally contains proteins of important physiological, antioxidant, and mucosal defense functions [such as albumin, immunoglobulin G (IgG), secretory IgA, transferrin, and ceruloplasmin]. Because concentrations of plasma proteins in alveolar fluid can increase in injured lungs (such as with permeability edema and inflammation), understanding how alveolar epithelium handles protein transport is needed to develop therapeutic measures to restore alveolar homeostasis. This… 
View on PubMed
ajplung.physiology.org
197 Citations
Highly Influential Citations
4
Background Citations
79
Methods Citations
5
Results Citations
7

Figures from this paper

197 Citations

Mechanisms of alveolar protein clearance in the intact lung.

Alveolar albumin clearance occurs by receptor-mediated transcytosis at low protein concentrations but proceeds by passive paracellular mechanisms at higher concentrations, and Because protein concentrations in pulmonary edema fluid are high, albumin concentrations of 5 g/100 ml or more, clearance of alveolar protein occurs byParacellular pathways in the setting of pulmonary edEMA.

Receptor-mediated endocytosis of macromolecules and strategy to enhance their transport in alveolar epithelial cells

Identification of the receptors involved in the cellular uptake of each macromolecule is prerequisite for the understanding and regulation of its transport into and across alveolar epithelial cells.

Claudins: Gatekeepers of lung epithelial function.

Albumin transcytosis across the epithelium of the lactating mouse mammary gland

Evidence is provided that albumin is transported across the mammary epithelium by the same pathway as immunoglobulin, and the possibility that the massive transfer of albumin into mouse milk is mediated by fluid phase transport is considered.

Transport of AOPP-Albumin into Human Alveolar Epithelial A549 Cell.

Alveolar clearance of AOPP-albumin is transported into alveolar epithelial cells through clathrin-mediated endocytosis, which may be important to prevent further ROS generation.

Critical Determinants of Uptake and Translocation of Nanoparticles by the Human Pulmonary Alveolar Epithelium

It is demonstrated that uptake and translocation across the pulmonary epithelium is controlled by alveolar type I epithelial cells, and furthermore, a number of factors that should be considered when designing new nanomedicines in order to improve drug delivery to the lung are highlighted.

TGF-β inhibits alveolar protein transport by promoting shedding, regulated intramembrane proteolysis, and transcriptional downregulation of megalin.

The molecular mechanisms by which transforming growth factor-β (TGF-β), a key molecule of ARDS pathogenesis, drives downregulation of megalin expression and function are investigated, suggesting that these MMPs may function as novel sheddases of meGalin.

Immunoglobulin Transport and Immunoglobulin Receptors

Characterization of protein factor(s) in rat bronchoalveolar lavage fluid that enhance insulin transport via transcytosis across primary rat alveolar epithelial cell monolayers.

Assessment of transport rates of proteins and peptides across primary human alveolar epithelial cell monolayers.

  • M. BurH. Huwer C. Ehrhardt
  • Biology
    European journal of pharmaceutical sciences : official journal of the European Federation for Pharmaceutical Sciences
  • 2006
...

References

SHOWING 1-10 OF 127 REFERENCES

Alveolar epithelial clearance of protein.

The present data suggest that most proteins and peptides deposited on the epithelial surfaces in the distal air spaces are cleared as intact molecules, predominantly via paracellular routes.

Differential permeability of endothelial and epithelial barriers to albumin flux.

  • A. GorinP. Stewart
  • Medicine, Biology
    Journal of applied physiology: respiratory, environmental and exercise physiology
  • 1979
It is found that alveolar lavage fluid collected by the technique is not significantly contaminated by plasma or interstitial fluid proteins, and not as a result of epithelial membrane damage occurring at the time of lavage.

Rates of protein transport across rat alveolar epithelial cell monolayers.

The transport of model proteins, ranging from 12,300 to 150,000 Da, across tight rat alveolar epithelial cell monolayers grown on polycarbonate filters, was studied and found that receptor-mediated or adsorptive transcytosis may be involved.

THE PERMEABILITY OF THE ALVEOLAR‐CAPILLARY MEMBRANE TO ULTRASTRUCTURAL PROTEIN TRACERS

  • E. Schneeberger
  • Biology, Medicine
    Annals of the New York Academy of Sciences
  • 1974
Those ultrastructural features of the alveolar-capillary membrane which enable it to act as a barrier to the diffusion of macromolecules are delineated and evidence that pinocytotic vesicles play a role in the bidirectional transport of proteins across this air-blood barrier is presented.

Evidence for the role of alveolar epithelial gp60 in active transalveolar albumin transport in the rat lung

Transcytosis of albumin, involving the 60 kDa albumin‐binding glycoprotein, gp60, was studied in cultured type II alveolar epithelial cells obtained from rat lungs, and cross‐linking of gp60 by antibody instillation stimulated only the displacement‐sensitive 125I‐labelled albumin transalveolar transport in intact rat lungs.

Ultrastructural evidence of transport of secretory IgA across bronchial epithelium.

These findings are consistent with a model for IgA transport in which IgA dimers are complexes with secretory component on the basolateral plasmalemma of epithelial cells in bronchial glands and transported across the cells in endocytic vesicles to the gland lumina.

Albumin absorption by canine bronchial epithelium.

The results demonstrate that albumin is absorbed by a low-affinity process that may contribute to the maintenance of low albumin concentrations in secretions.

Size-dependent dextran transport across rat alveolar epithelial cell monolayers.

Equivalent pore analysis, assuming restricted diffusion of FDs of 4-40 kDa via cylindrical, water-filled pores across the cell monolayer revealed a population of large equivalent pores with approximately 5.6 nm radius, suggesting that smaller macromolecules traverse the alveolar epithelial barrier via paracellular pathways, and that larger macromolescules likely cross the barrier via other pathways.

Protein permeability in the adult respiratory distress syndrome. Loss of size selectivity of the alveolar epithelium.

It is concluded that normal size selectivity is preserved in cardiac edema but is destroyed by the alveolar-capillary injury of ARDS.

Bidirectional FcRn-dependent IgG transport in a polarized human intestinal epithelial cell line.

A novel bidirectional mechanism of IgG transport across epithelial barriers that predicts an important effect of FcRn on IgG function in immune surveillance and host defense at mucosal surfaces is defined.
...