Protein transport across membranes: Comparison between lysine and guanidinium-rich carriers.

@article{Lein2015ProteinTA,
  title={Protein transport across membranes: Comparison between lysine and guanidinium-rich carriers.},
  author={Max Lein and Brittany M Deronde and Federica Sgolastra and Gregory N Tew and Matthew Alexander Holden},
  journal={Biochimica et biophysica acta},
  year={2015},
  volume={1848 11 Pt A},
  pages={
          2980-4
        }
}
The mechanism(s) by which certain small peptides and peptide mimics carry large cargoes across membranes through exclusively non-covalent interactions has been difficult to resolve. Here, we use the droplet-interface bilayer as a platform to characterize distinct mechanistic differences between two such carriers: Pep-1 and a guanidinium-rich peptide mimic we call D9. While both Pep-1 and D9 can carry an enzyme, horseradish peroxidase (HRP) across a lipid bilayer, we found that they do so by… CONTINUE READING

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