Protein translocation across the endoplasmic reticulum membrane: identification by photocross-linking of a 39-kD integral membrane glycoprotein as part of a putative translocation tunnel

@article{Krieg1989ProteinTA,
  title={Protein translocation across the endoplasmic reticulum membrane: identification by photocross-linking of a 39-kD integral membrane glycoprotein as part of a putative translocation tunnel},
  author={U C Krieg and Arthur E. Johnson and Peter J. Walter},
  journal={The Journal of Cell Biology},
  year={1989},
  volume={109},
  pages={2033 - 2043}
}
The molecular environment of secretory proteins during translocation across the ER membrane was examined by photocross-linking. Nascent preprolactin chains of various lengths, synthesized by in vitro translation of truncated messenger RNAs in the presence of N epsilon-(5-azido-2-nitrobenzoyl)-Lys-tRNA, signal recognition particle, and microsomal membranes… CONTINUE READING