Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase

@article{Harding1999ProteinTA,
  title={Protein translation and folding are coupled by an endoplasmic-reticulum-resident kinase},
  author={Heather P. Harding and Yuhong Zhang and David Ron},
  journal={Nature},
  year={1999},
  volume={397},
  pages={271-274}
}
Protein synthesis and the folding of the newly synthesized proteins into the correct three-dimensional structure are coupled in cellular compartments of the exocytosis pathway by a process that modulates the phosphorylation level of eukaryotic initiation factor-2α (eIF2α) in response to a stress signal from the endoplasmic reticulum (ER),. Activation of this process leads to reduced rates of initiation of protein translation during ER stress. Here we describe the cloning of perk, a gene… Expand
A Novel Feedback Loop Regulates the Response to Endoplasmic Reticulum Stress via the Cooperation of Cytoplasmic Splicing and mRNA Translation
TLDR
A model in which eIF2α phosphorylation-mediated control of mRNA turnover is a molecular switch that regulates the stress response transcription program and the ER's capacity for protein folding during stress is proposed. Expand
Activation-dependent substrate recruitment by the eukaryotic translation initiation factor 2 kinase PERK
TLDR
It is reported that PERK activation and phosphorylation selectively enhance its affinity for the nonphosphorylated eIF2 complex, and this switch correlates with a marked change to the protease sensitivity pattern, indicative of a major conformational change in the PERK kinase domain upon activation. Expand
The double-stranded RNA-activated protein kinase PKR is dispensable for regulation of translation initiation in response to either calcium mobilization from the endoplasmic reticulum or essential amino acid starvation.
TLDR
The results show that PKR is not required for increased eIF2alpha phosphorylation or inhibition of protein synthesis under conditions promoting the UPR or in response to amino acid deprivation. Expand
Phosphorylation of Eukaryotic Translation Initiation Factor 2α Coordinates rRNA Transcription and Translation Inhibition during Endoplasmic Reticulum Stress
TLDR
The UPR downregulates the synthesis of rRNA by inactivation of the RNA polymerase I basal transcription factor RRN3/TIF-IA, suggesting that this pathway may be broadly utilized by stresses that activate eIF2α kinases in order to coordinately regulate translation and ribosome biogenesis during cellular stress. Expand
The structure of the PERK kinase domain suggests the mechanism for its activation.
TLDR
The crystal structure of PERK's kinase domain suggests conservation in the mode of activation of eIF2α kinases and is consistent with a `line-up' model for PERK activation triggered by oligomerization of its luminal domain. Expand
The Protein Kinase/Endoribonuclease IRE1α That Signals the Unfolded Protein Response Has a Luminal N-terminal Ligand-independent Dimerization Domain*
TLDR
The N-terminal luminal domains of IRE1 and PERK sense ER stress conditions by a common mechanism and transmit the signal to regulate the cytoplasmic domains of these receptors, and it is demonstrated that the NLD forms stable dimers linked by intermolecular disulfide bridges. Expand
Mammalian transcription factor ATF6 is synthesized as a transmembrane protein and activated by proteolysis in response to endoplasmic reticulum stress.
TLDR
It is proposed that ER stress-induced proteolysis of membrane-bound p90 ATF6 releases soluble p50ATF6, leading to induced transcription in the nucleus, similar to that reported for cholesterol homeostasis. Expand
Regulation of Sumo mRNA during Endoplasmic Reticulum Stress
TLDR
It is shown that Smt3, a homolog of small ubiquitin-like modifier (sumo), is a non-canonical RIDD target in Drosophila S2 cells and unlike other RIDd targets, the sumo transcript does not stably associate with the ER membrane, but instead relies on an Xbp1-like stem loop and a second UPR mediator, Perk, for its degradation during stress. Expand
Nck in a Complex Containing the Catalytic Subunit of Protein Phosphatase 1 Regulates Eukaryotic Initiation Factor 2α Signaling and Cell Survival to Endoplasmic Reticulum Stress*
TLDR
It is demonstrated that the Src homology (SH) domain-containing adaptor Nck is a key component of a molecular complex that controls eIF2α phosphorylation and signaling in response to ER stress and contributes to maintain eif2α in a hypophosphorylated state. Expand
Modulation of the Eukaryotic Initiation Factor 2 α-Subunit Kinase PERK by Tyrosine Phosphorylation*
TLDR
PerK is classified as a dual specificity kinase whose regulation by tyrosine phosphorylation contributes to its optimal activation in response to ER stress. Expand
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