Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803.

@article{Wu1998ProteinTB,
  title={Protein trans-splicing by a split intein encoded in a split DnaE gene of Synechocystis sp. PCC6803.},
  author={H Wu and Z Hu and X Q Liu},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1998},
  volume={95 16},
  pages={9226-31}
}
  • H Wu, Z Hu, X Q Liu
  • Published 1998 in
    Proceedings of the National Academy of Sciences…
A split intein capable of protein trans-splicing is identified in a DnaE protein of the cyanobacterium Synechocystis sp. strain PCC6803. The N- and C-terminal halves of DnaE (catalytic subunit alpha of DNA polymerase III) are encoded by two separate genes, dnaE-n and dnaE-c, respectively. These two genes are located 745,226 bp apart in the genome and on opposite DNA strands. The dnaE-n product consists of a N-extein sequence followed by a 123-aa intein sequence, whereas the dnaE-c product… CONTINUE READING
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Wu et al

  • H. Wu, Xu, M.-Q., Liu X.-Q. Biochim. Biophys. Acta, in press. Biochemistry
  • Proc. Natl. Acad. Sci. USA 95
  • 1998

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