Protein targeting across the three membranes of the Euglena chloroplast envelope.

@article{Shashidhara1992ProteinTA,
  title={Protein targeting across the three membranes of the Euglena chloroplast envelope.},
  author={L S Shashidhara and Shu Hui Lim and Jamie B. Shackleton and Colin G. Robinson and Alison G Smith},
  journal={The Journal of biological chemistry},
  year={1992},
  volume={267 18},
  pages={
          12885-91
        }
}
A system has been developed for the import in vitro of precursor proteins into Euglena chloroplasts, which have three envelope membranes. Preparation of functional chloroplasts with intact envelope membranes has been optimized. Import of the precursor (50 kDa) for the tetrapyrrole biosynthesis enzyme porphobilinogen deaminase (PBGD), and processing to the mature size (40 kDa), occurred at 25 degrees C in the light and the presence of ATP, with an estimated efficiency of 62%. Pretreatment of the… CONTINUE READING

Citations

Publications citing this paper.
SHOWING 1-10 OF 14 CITATIONS

Similar Papers

Loading similar papers…