Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein

@article{Cabantous2005ProteinTA,
  title={Protein tagging and detection with engineered self-assembling fragments of green fluorescent protein},
  author={S. Cabantous and T. Terwilliger and G. Waldo},
  journal={Nature Biotechnology},
  year={2005},
  volume={23},
  pages={102-107}
}
Existing protein tagging and detection methods are powerful but have drawbacks. Split protein tags can perturb protein solubility or may not work in living cells. Green fluorescent protein (GFP) fusions can misfold or exhibit altered processing. Fluorogenic biarsenical FLaSH or ReASH substrates overcome many of these limitations but require a polycysteine tag motif, a reducing environment and cell transfection or permeabilization. An ideal protein tag would be genetically encoded, would work… Expand
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