Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts.

@article{BenZeev1980ProteinSR,
  title={Protein synthesis requires cell-surface contact while nuclear events respond to cell shape in anchorage-dependent fibroblasts.},
  author={Avri Ben-Ze'ev and Stephen R. Farmer and Sheldon Penman},
  journal={Cell},
  year={1980},
  volume={21 2},
  pages={365-72}
}
Anchorage-dependent mouse fibroblasts grow only if attached to and spread on a solid substrate. The suspension of cells in methyl-cellulose results in dramatic, coordinated inhibition of the major RNA and protein synthesis systems, and these systems are sequentially restored when cells are replated on a tissue culture dish surface. In the present report the effects on metabolism of cell reattachment are separated from those of subsequent spreading by controlling cell shape. Macromolecular… CONTINUE READING