Protein synthesis by astrocytes in primary cultures

@article{White1981ProteinSB,
  title={Protein synthesis by astrocytes in primary cultures},
  author={Fredric P. White and Leif Hertz},
  journal={Neurochemical Research},
  year={1981},
  volume={6},
  pages={353-364}
}
Protein synthesis, measured as leucine incorporation into acid-precipitable proteins, was determined in astrocytes in primary cultures obtained from the cerebral hemispheres of newborn mice. As can be expected for eucaryotic, ribosomal protein synthesis, the incorporation was almost completely inhibited by cycloheximide (0.01 mM), but unaffected by chloramphenicol (0.03 mM). The rate of synthesis, measured during exposure to a high (0.8 mM) concentration of leucine was 5.4 nmol/hr/mg protein in… CONTINUE READING
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