Protein structure in KBr pellets by infrared spectroscopy.

  title={Protein structure in KBr pellets by infrared spectroscopy.},
  author={Lucimara Aparecida Forato and Rubens Bernardes-Filho and Luiz Alberto Colnago},
  journal={Analytical biochemistry},
  volume={259 1},
In this work we analyzed the secondary structure of 13 globular proteins in KBr pellet through Fourier transform infrared spectroscopy (FTIR). The quantification was based in singular value decomposition (SVD) theory, a pattern recognition method. The results show better correlation for alpha helix (0.90) and beta sheet (0.84) in amide I band, similar to the results obtained for proteins in solution. These results show that the protein secondary structure is conserved in solid state, in… CONTINUE READING