Protein structure determination by MicroED.

@article{Nannenga2014ProteinSD,
  title={Protein structure determination by MicroED.},
  author={Brent L. Nannenga and Tamir Gonen},
  journal={Current opinion in structural biology},
  year={2014},
  volume={27},
  pages={
          24-31
        }
}
In this review we discuss the current advances relating to structure determination from protein microcrystals with special emphasis on the newly developed method called MicroED. This method uses a transmission electron cryo-microscope to collect electron diffraction data from extremely small 3-dimensional (3D) crystals. MicroED has been used to solve the 3D structure of the model protein lysozyme to 2.9Å resolution. As the method further matures, MicroED promises to offer a unique and widely… Expand
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The use of X-ray crystallography for the structure determination of biological macromolecules has experienced a steady expansion over the last 20 years with the Protein Data Bank growing from 100 000Expand
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