Protein structure by mechanical triangulation.

  title={Protein structure by mechanical triangulation.},
  author={Hendrik Dietz and Matthias Rief},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  volume={103 5},
Knowledge of protein structure is essential to understand protein function. High-resolution protein structure has so far been the domain of ensemble methods. Here, we develop a simple single-molecule technique to measure spatial position of selected residues within a folded and functional protein structure in solution. Construction and mechanical unfolding of cysteine-engineered polyproteins with controlled linkage topology allows measuring intramolecular distance with angstrom precision. We… CONTINUE READING