Protein stitchery: design of a protein for selective binding to a specific DNA sequence.

@article{Park1992ProteinSD,
  title={Protein stitchery: design of a protein for selective binding to a specific DNA sequence.},
  author={Changmoon Park and Judy L. Campbell and William A. Goddard},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1992},
  volume={89 19},
  pages={9094-6}
}
We present a general strategy for designing proteins to recognize DNA sequences and illustrate this with an example based on the "Y-shaped scissors grip" model for leucine-zipper gene-regulatory proteins. The designed protein is formed from two copies, in tandem, of the basic (DNA binding) region of v-Jun. These copies are coupled through a tripeptide to yield a "dimer" expected to recognize the sequence TCATCGATGA (the v-Jun-v-Jun homodimer recognizes ATGACTCAT). We synthesized the protein and… CONTINUE READING

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