Protein staining of ribboned epon sections for light microscopy

@article{Fisher1968ProteinSO,
  title={Protein staining of ribboned epon sections for light microscopy},
  author={Donald B. Fisher},
  journal={Histochemie},
  year={1968},
  volume={16},
  pages={92-96}
}
Procedures are described for obtaining and handling ribboned epon sections 0.3–2 μ thick for light microscopy, and for the cytological application of two intense acid dyes, Aniline Blue Black and Coomassie Brilliant Blue R 250. The technique allows precise localization of proteins and some other materials, and, because the sections are ribboned, facilitates three-dimensional visualization of the structures involved. The dyes may be used in combination with the periodic acid-Schiff reaction and… CONTINUE READING
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