Protein purification using combined streptavidin (or avidin)-Sepharose and thiopropyl-Sepharose affinity chromatography.

Abstract

The major problem usually encountered in the application of the (strept)avidin-biotin system to the purification of proteins (or other biological molecules) lies in the difficult reversion of the interaction between immobilized (strept)avidin and the adsorbed biotinylated protein. Among the proposed solutions is the selective biotinylation of the entity to… (More)

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Cite this paper

@article{Desarnaud1992ProteinPU, title={Protein purification using combined streptavidin (or avidin)-Sepharose and thiopropyl-Sepharose affinity chromatography.}, author={F Desarnaud and J M{\'a}rie and R Larguier and Catherine B. Lombard and S. Jard and Jean C Bonnafous}, journal={Journal of chromatography}, year={1992}, volume={603 1-2}, pages={95-104} }