Protein-protein interactions of the primase subunits p58 and p48 with simian virus 40 T antigen are required for efficient primer synthesis in a cell-free system.

@article{Weisshart2000ProteinproteinIO,
  title={Protein-protein interactions of the primase subunits p58 and p48 with simian virus 40 T antigen are required for efficient primer synthesis in a cell-free system.},
  author={Klaus Weisshart and Helmwart F{\"o}rster and Elisabeth Kremmer and Bernhard Schlott and F. Grosse and H-P. Nasheuer},
  journal={The Journal of biological chemistry},
  year={2000},
  volume={275 23},
  pages={
          17328-37
        }
}
DNA polymerase alpha-primase (pol-prim, consisting of p180-p68-p58-p48), and primase p58-p48 (prim(2)) synthesize short RNA primers on single-stranded DNA. In the SV40 DNA replication system, only pol-prim is able to start leading strand DNA replication that needs unwinding of double-stranded (ds) DNA prior to primer synthesis. At high concentrations, pol-prim and prim(2) indistinguishably reduce the unwinding of dsDNA by SV40 T antigen (Tag). RNA primer synthesis on ssDNA in the presence of… CONTINUE READING
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