Protein-protein interactions in calcium transport regulation probed by saturation transfer electron paramagnetic resonance.

@article{James2012ProteinproteinII,
  title={Protein-protein interactions in calcium transport regulation probed by saturation transfer electron paramagnetic resonance.},
  author={Zachary M James and Jesse E McCaffrey and Kurt D Torgersen and Christine B. Karim and David D Thomas},
  journal={Biophysical journal},
  year={2012},
  volume={103 6},
  pages={1370-8}
}
We have used electron paramagnetic resonance (EPR) to probe the homo- and heterooligomeric interactions of reconstituted sarcoplasmic reticulum Ca-ATPase (SERCA) and its regulator phospholamban (PLB). SERCA is responsible for restoring calcium to the sarcoplasmic reticulum to allow muscle relaxation, whereas PLB inhibits cardiac SERCA unless phosphorylated at Ser(16). To determine whether changes in protein association play essential roles in regulation, we detected the microsecond rotational… CONTINUE READING

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