Protein-protein interactions in calcium transport regulation probed by saturation transfer electron paramagnetic resonance.

@article{James2012ProteinproteinII,
  title={Protein-protein interactions in calcium transport regulation probed by saturation transfer electron paramagnetic resonance.},
  author={Zachary M James and Jesse E McCaffrey and Kurt D Torgersen and Christine B. Karim and David D Thomas},
  journal={Biophysical journal},
  year={2012},
  volume={103 6},
  pages={1370-8}
}
We have used electron paramagnetic resonance (EPR) to probe the homo- and heterooligomeric interactions of reconstituted sarcoplasmic reticulum Ca-ATPase (SERCA) and its regulator phospholamban (PLB). SERCA is responsible for restoring calcium to the sarcoplasmic reticulum to allow muscle relaxation, whereas PLB inhibits cardiac SERCA unless phosphorylated… CONTINUE READING