Protein-protein- and protein-RNA-binding properties of the movement protein and VP25 coat protein of Apple latent spherical virus.

@article{Isogai2006ProteinproteinAP,
  title={Protein-protein- and protein-RNA-binding properties of the movement protein and VP25 coat protein of Apple latent spherical virus.},
  author={Masamichi Isogai and Kentaro Watanabe and Yusuke Uchidate and Nobuyuki Yoshikawa},
  journal={Virology},
  year={2006},
  volume={352 1},
  pages={
          178-87
        }
}
To elucidate the mechanism of Apple latent spherical virus (ALSV) movement, various properties of its cell-to-cell movement protein (MP) were analyzed. ELISA and blot overlay assays demonstrated that the MP bound specifically to ALSV virions and in particular to one of the three coat proteins (VP25) but not to the other two coat proteins (VP20 and VP24). Mutational analyses have revealed that the MP contains two domains with independent VP25-binding activity (amino acid residues 1-188 and 189… CONTINUE READING

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