Protein profile of exosomes from trabecular meshwork cells.

@article{Stamer2011ProteinPO,
  title={Protein profile of exosomes from trabecular meshwork cells.},
  author={William Daniel Stamer and Emely A Hoffman and James Matthew Luther and David L. Hachey and Kevin L. Schey},
  journal={Journal of proteomics},
  year={2011},
  volume={74 6},
  pages={796-804}
}
To better understand the role of exosomes in the trabecular meshwork (TM), the site of intraocular pressure control, the exosome proteome from primary cultures of human TM cell monolayers was analyzed. Exosomes were purified from urine and conditioned media from primary cultures of human TM cell monolayers and subjected to a two dimensional HPLC separation and MS/MS analyses using the MudPIT strategy. Spectra were searched against a human protein database using Sequest. Protein profiles were… CONTINUE READING

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