Protein phosphorylation during coconut zygotic embryo development

  title={Protein phosphorylation during coconut zygotic embryo development},
  author={Islas-Flores and Oropeza and Hernandez-Sotomayor},
  journal={Plant physiology},
  volume={118 1},
Evidence was obtained on the occurrence of protein threonine, serine, and tyrosine (Tyr) kinases in developing coconut (Cocos nucifera L. ) zygotic embryos, based on in vitro phosphorylation of proteins in the presence of [gamma-32P]ATP, alkaline treatment, and thin-layer chromatography analysis, which showed the presence of [32P]phosphoserine, [32P]phosphothreonine, and [32P]phosphotyrosine in [32P]-labeled protein hydrolyzates. Tyr kinase activity was further confirmed in extracts of embryos… CONTINUE READING
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