Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter.

@article{Dahl1997ProteinPA,
  title={Protein phosphorylation affects binding of the Escherichia coli transcription activator UhpA to the uhpT promoter.},
  author={Jacob L. Dahl and B Y Wei and Robert J. Kadner},
  journal={The Journal of biological chemistry},
  year={1997},
  volume={272 3},
  pages={1910-9}
}
Expression of the Escherichia coli sugar phosphate transporter UhpT is induced by extracellular glucose 6-phosphate through a transmembrane signaling process dependent on the sensor kinase UhpB and the UhpT homolog, UhpC. These proteins are thought to regulate the phosphorylation of the transcription activator, UhpA. To examine the effect of protein phosphorylation on the binding of UhpA to target sequences in the uhpT promoter region, the UhpA protein was overexpressed and purified. Purified… CONTINUE READING