Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stress.

@article{Bogaart2007ProteinMA,
  title={Protein mobility and diffusive barriers in Escherichia coli: consequences of osmotic stress.},
  author={Geert van den Bogaart and Nicolaas Hermans and Victor V. Krasnikov and Bert Poolman},
  journal={Molecular microbiology},
  year={2007},
  volume={64 3},
  pages={858-71}
}
The effect of osmotic stress on the intracellular diffusion of proteins in Escherichia coli was studied, using a pulsed version of fluorescence recovery after photo-bleaching, pulsed-FRAP. This method employs sequences of laser pulses which only partly bleach the fluorophores in a cell. Because the cell size and geometry are taken into account, pulsed-FRAP enables to measure diffusion in very small cells of different shapes. We found that upon an osmotic upshock from 0.15 to 0.6 Osm, imposed by… CONTINUE READING

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