Protein measurement with the Folin phenol reagent.

  title={Protein measurement with the Folin phenol reagent.},
  author={O. H. Lowry and N. J. Rosebrough and A. Farr and R. Randall},
  journal={The Journal of biological chemistry},
  volume={193 1},
  • O. H. Lowry, N. J. Rosebrough, +1 author R. Randall
  • Published 1951
  • Chemistry, Medicine
  • The Journal of biological chemistry
  • Since 1922 when Wu proposed the use of the Folin phenol reagent for the measurement of proteins, a number of modified analytical procedures utilizing this reagent have been reported for the determination of proteins in serum, in antigen-antibody precipitates, and in insulin. Although the reagent would seem to be recommended by its great sensitivity and the simplicity of procedure possible with its use, it has not found great favor for general biochemical purposes. In the belief that this… CONTINUE READING
    274,654 Citations

    Topics from this paper

    Determination of proteins and sulfobetaine with the Folin-phenol reagent.
    • M. Ledoux, F Lamy
    • Chemistry, Medicine
    • Analytical biochemistry
    • 1986
    • 80
    A new colorimetric technique for the estimation of vitamin C using Folin phenol reagent.
    • 392
    • 54
    Review of the Folin phenol protein quantitation method of Lowry, Rosebrough, Farr and Randall.
    • G. Peterson
    • Chemistry, Medicine
    • Analytical biochemistry
    • 1979
    • 1,054
    Simple Microassay of Protein with Membrane Filter
    • 99
    Measurement and characterization of proteins by color reactions.
    • 23