Protein localization in living cells and tissues using FRET and FLIM.

  title={Protein localization in living cells and tissues using FRET and FLIM.},
  author={Ye Chen and James D. Mills and Ammasi Periasamy},
  journal={Differentiation; research in biological diversity},
  volume={71 9-10},
Interacting proteins assemble into molecular machines that control cellular homeostasis in living cells. While the in vitro screening methods have the advantage of providing direct access to the genetic information encoding unknown protein partners, they do not allow direct access to interactions of these protein partners in their natural environment inside the living cell. Using wide-field, confocal, or two-photon (2p) fluorescence resonance energy transfer (FRET) microscopy, this information… CONTINUE READING


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