Protein-liposome conjugates using cysteine-lipids and native chemical ligation.

  title={Protein-liposome conjugates using cysteine-lipids and native chemical ligation.},
  author={Sanne W A Reulen and Wilco W T Brusselaars and Sander Langereis and Willem J M Mulder and Monica Breurken and Maarten Merkx},
  journal={Bioconjugate chemistry},
  volume={18 2},
Liposomes have become popular drug delivery vehicles and have more recently also been applied as contrast agents for molecular imaging. Most current methods for functionalization of liposomes with targeting proteins rely on reactions of amine or thiol groups at the protein exterior, which generally result in nonspecific conjugation at multiple sites on the protein. In this study, we present native chemical ligation (NCL) as a general method to covalently couple recombinant proteins in a highly… CONTINUE READING
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