Protein-lipid interactions with Fusobacterium nucleatum major outer membrane protein FomA: spin-label EPR and polarized infrared spectroscopy.

@article{Anbazhagan2008ProteinlipidIW,
  title={Protein-lipid interactions with Fusobacterium nucleatum major outer membrane protein FomA: spin-label EPR and polarized infrared spectroscopy.},
  author={Veerappan Anbazhagan and Natarajan Vijay and J{\"o}rg H. Kleinschmidt and Derek Marsh},
  journal={Biochemistry},
  year={2008},
  volume={47 32},
  pages={8414-23}
}
FomA, the major outer membrane protein of Fusobacterium nucleatum, was expressed and purified in Escherichia coli and reconstituted from detergent in bilayer membranes of phosphatidylcholines with chain lengths from C(12:0) to C(17:0). The conformation and orientation of membrane-incorporated FomA were determined from polarized, attenuated total reflection, infrared (IR) spectroscopy, and lipid-protein interactions with FomA were characterized by using electron paramagnetic resonance (EPR… CONTINUE READING

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