Protein-lipid interactions in antipodal plasma membranes of rat colonocytes.

@article{Brasitus1984ProteinlipidII,
  title={Protein-lipid interactions in antipodal plasma membranes of rat colonocytes.},
  author={Thomas A. Brasitus and Roger S. Keresztes},
  journal={Biochimica et biophysica acta},
  year={1984},
  volume={773 2},
  pages={
          290-300
        }
}
The isolation of apical membranes from rat proximal colonic epithelial cells is described. Differential centrifugation yielded a 'crude' membrane fraction which was further purified using sucrose density centrifugation. The final membrane fraction was enriched 20-28-fold over homogenate in alkaline phosphatase and cysteine-sensitive alkaline phosphatase specific activities. Lipid-protein interactions and lipid dynamics examined in apical and basolateral membranes prepared from colonocytes… CONTINUE READING
BETA

Citations

Publications citing this paper.
SHOWING 1-10 OF 12 CITATIONS

Similar Papers

Loading similar papers…