Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.

Abstract

Antibodies raised against the protein encoded by a lacZ-CDC28 in-frame fusion were shown to immunoprecipitate the CDC28 product from yeast cell lysates. The polypeptide p36CDC28 is a phosphoprotein of apparent Mr 36,000. Immune complexes prepared from yeast cell lysates by using anti-CDC28 antibody were found to possess a protein kinase activity, as determined by the transfer of label from [gamma-32P]ATP to a coprecipitated Mr 40,000 protein of unknown identity or function (p40). This activity was absent or thermolabile when extracts were prepared from several different cdc28 temperature-sensitive strains. The protein kinase activity was dependent on Zn2+ and transferred phosphate specifically to serine and threonine residues.

050'89'92'95'98'01'04'07'10'13'16
Citations per Year

556 Citations

Semantic Scholar estimates that this publication has 556 citations based on the available data.

See our FAQ for additional information.

Cite this paper

@article{Reed1985ProteinKA, title={Protein kinase activity associated with the product of the yeast cell division cycle gene CDC28.}, author={S. I. Reed and J. A. Hadwiger and Andr{\'a}s L{\"{o}rincz}, journal={Proceedings of the National Academy of Sciences of the United States of America}, year={1985}, volume={82 12}, pages={4055-9} }