Protein kinase G phosphorylates mosquito-borne flavivirus NS5.

  title={Protein kinase G phosphorylates mosquito-borne flavivirus NS5.},
  author={Dipankar Bhattacharya and Mayuri and Sonja M. Best and Rushika Perera and Richard J. Kuhn and Rob Striker},
  journal={Journal of virology},
  volume={83 18},
Serine/threonine phosphorylation of the nonstructural protein 5 (NS5) is a conserved feature of flaviviruses, but the kinase(s) responsible and function(s) remain unknown. Mass spectrometry was used to compare the phosphorylation sites of the NS5 proteins of yellow fever virus (YFV) and dengue virus (DENV), two flaviviruses transmitted by mosquitoes. Seven DENV phosphopeptides were identified, but only one conserved phosphoacceptor site (threonine 449 in DENV) was identified in both viruses… CONTINUE READING