Protein kinase D mediates the synergistic effects of BMP-7 and IGF-I on osteoblastic cell differentiation.

@article{Yeh2010ProteinKD,
  title={Protein kinase D mediates the synergistic effects of BMP-7 and IGF-I on osteoblastic cell differentiation.},
  author={L C Yeh and Xiuye Ma and Ronald W. Matheny and Martin L. Adamo and John C. H. Lee},
  journal={Growth factors},
  year={2010},
  volume={28 5},
  pages={318-28}
}
We previously showed that exogenous insulin-like growth factor-I (IGF-I) and bone morphogenetic protein-7 (BMP-7) synergistically stimulated osteoblast differentiation in fetal rat calvaria (FRC) cells. We have now shown that BMP-7 alone and the BMP-7 and IGF-I combination synergistically stimulated protein kinase D (PKD) phosphorylation at Ser744/748 and Ser916. Transfection of FRC cells with a constitutively active PKD stimulated marker expression, while transfection with a catalytically… CONTINUE READING