Protein kinase C regulates keratinocyte transglutaminase (TGK) gene expression in cultured primary mouse epidermal keratinocytes induced to terminally differentiate by calcium.

@article{Dlugosz1994ProteinKC,
  title={Protein kinase C regulates keratinocyte transglutaminase (TGK) gene expression in cultured primary mouse epidermal keratinocytes induced to terminally differentiate by calcium.},
  author={Andrzej A. Dlugosz and Stuart H. Yuspa},
  journal={The Journal of investigative dermatology},
  year={1994},
  volume={102 4},
  pages={
          409-14
        }
}
  • A. Dlugosz, S. Yuspa
  • Published 1 April 1994
  • Biology, Medicine
  • The Journal of investigative dermatology
During the final stage of epidermal differentiation, activation of keratinocyte transglutaminase results in covalent crosslinking of a variety of proteins to form highly protective cornified cell envelopes. We have studied the regulation of keratinocyte transglutaminase (TGK) gene expression in murine epidermal keratinocytes induced to terminally differentiate in vitro by increasing the level of extracellular Ca++ or treatment with the protein kinase C (PKC) activator 12-O-tetradecanoylphorbol… Expand
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References

SHOWING 1-10 OF 60 REFERENCES
Regulation of transglutaminase type I expression in squamous differentiating rabbit tracheal epithelial cells and human epidermal keratinocytes: effects of retinoic acid and phorbol esters.
TLDR
The full length cDNA sequence for rabbit transglutaminase type I as well as the sequence for a 2.9-kilobase (kb) promoter fragment of the gene are described, indicating that transglUTaminasetype I mRNA expression was associated with differentiation and not growth arrest. Expand
Coordinate changes in gene expression which mark the spinous to granular cell transition in epidermis are regulated by protein kinase C
TLDR
The results suggest that PKC is a fundamental regulator of the coordinate changes in keratinocyte gene expression that occur during the spinous to granular cell transition in epidermis. Expand
Expression of murine epidermal differentiation markers is tightly regulated by restricted extracellular calcium concentrations in vitro
TLDR
In vitro results suggest that the Ca2+ environment is a fundamental regulator of expression of epidermal differentiation markers and provide an explanation for the existence of theCa2+ gradient in vivo. Expand
Protein kinase C in normal human epidermal keratinocytes during proliferation and calcium-induced differentiation.
TLDR
PKC in NHEK appears to be upregulated under conditions of Ca(++)-induced growth arrest and differentiation, and immunoreactive protein levels are higher than normal under these conditions. Expand
Early signals for keratinocyte differentiation: role of Ca2+-mediated inositol lipid metabolism in normal and neoplastic epidermal cells.
TLDR
The results demonstrate that the second messenger system for Ca2+-mediated keratinocyte differentiation may be through a direct effect on phospholipase C activity, and suggest that both protein kinase C activation, elevation of intracellular calcium and PIP turnover were important components of the signal for epidermal differentiation. Expand
Regulation of transglutaminase 1 gene expression by 12-O-tetradecanoylphorbol-13-acetate, dexamethasone, and retinoic acid in cultured human keratinocytes.
TLDR
It is suggested that the expression of TG1 gene may be upregulated by protein kinase C and glucocorticoid receptor systems and down-regulated by the retinoic acid receptor system. Expand
Modulation of tissue and epidermal transglutaminases in mouse epidermal cells after treatment with 12-O-tetradecanoylphorbol-13-acetate and/or retinoic acid in vivo and in culture.
TLDR
The results are consistent with RA causing a reprogramming of epidermal cells that alters their response to differentiation stimuli and the increase in tissue TGASE due to RA was markedly inhibited by TPA. Expand
Type I keratinocyte transglutaminase: expression in human skin and psoriasis.
TLDR
Quantitative LSCM provides a powerful and direct method for analysis of gene expression in skin and quantitative Northern blot analysis of TGase K mRNA from normal and involved epidermal biopsies from psoriasis patients suggests that TGaseK mRNA levels are increased in psoriatic lesions. Expand
Calcium regulation of growth and differentiation of mouse epidermal cells in culture
TLDR
Ulastructural examination of cells cultured under low Ca++ conditions reveals widened intercellular spaces, abundant microvilli and perinuclear organization of tonofilaments and cellular organelles. Expand
Transcripts encoding protein kinase C‐α, ‐ϵ, ‐ζ, and ‐ζ are expressed in basal and differentiating mouse keratinocytes in vitro and exhibit quantitative changes in neoplastic cells
TLDR
Interestingly, the PKC‐ζ transcripts detected in RNA isolated from keratinocytes were approximately 200 nucleotides longer than those from mouse brain, suggesting the existence of an alternative form of this isozyme. Expand
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