Protein kinase C-ε protects MCF-7 cells from TNF-mediated cell death by inhibiting Bax translocation

@article{Lu2007ProteinKC,
  title={Protein kinase C-$\epsilon$ protects MCF-7 cells from TNF-mediated cell death by inhibiting Bax translocation},
  author={Dongmei Lu and Usha Sivaprasad and Jie Huang and Eswar Shankar and Shavonda Morrow and Alakananda Basu},
  journal={Apoptosis},
  year={2007},
  volume={12},
  pages={1893-1900}
}
We have previously shown that protein kinase Cε (PKCε) acts as an antiapoptotic protein and protects breast cancer MCF-7 cells from tumor necrosis factor-α (TNF)-mediated apoptosis. In the present study, we have investigated the mechanism by which PKCε inhibits TNF-induced cell death. Overexpression of wild-type PKCε (WT-PKCε) in MCF-7 cells decreased TNF-induced mitochondrial depolarization. Depletion of Bax by small interfering RNA (siRNA) attenuated TNF-induced cell death. Overexpression of… 

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