Protein interaction platforms: visualization of interacting proteins in yeast

Abstract

Here we describe the protein interaction platform assay, a method for identifying interacting proteins in Saccharomyces cerevisiae. This assay relies on the reovirus scaffolding protein μNS, which forms large focal inclusions in living cells. When a query protein is fused to μNS and potential interaction partners are fused to a fluorescent reporter, interactors can be identified by screening for yeast that display fluorescent foci.

DOI: 10.1038/nmeth.1337

3 Figures and Tables

Cite this paper

@article{Schmitz2009ProteinIP, title={Protein interaction platforms: visualization of interacting proteins in yeast}, author={Alexa M. Schmitz and Monica F Morrison and Akochi O Agunwamba and Max L Nibert and Cammie F. Lesser}, journal={Nature Methods}, year={2009}, volume={6}, pages={500-502} }